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By applying the multiple normal tissues corrected differential analysis, we identified some genes as novel biomarker candidates.
Expression of CA125/MUC16 in multiple normal tissues and in different pathological and physiological conditions implies a broad biological role for CA125/MUC16 [1].
RNA-sequencing data for 6757 samples from The Cancer Genome Atlas were analyzed to characterize ELF5 isoform expression in multiple normal tissues and cancers.
Figure 3 shows the expression profiles across multiple normal tissues of five disease genes known to underlie retinitis pigmentosa, while Tables 2 and 3 show the summary of the mean ranks obtained using our method.
Thus, a single-cell approach can help to definitively reconstruct the cellular composition of tumours as has been effectively carried out for multiple normal tissues using single-cell transcriptomics techniques (20, 56– 61).
Therefore, additional in vivo experiments in which the therapeutic role of nutlin-3 is assessed on multiple normal tissues in human tumor xenografts with different p53 status may help to better understand the clinical potential of this approach.
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For a straightforward validation of subclass mapping, we used two data sets of multiple normal tissue types acquired on two different generations of the Affymetrix GeneChip oligonucleotide microarray platform, Multi-A [5] and Multi-B [6].
Further investigation involving multiple normal tissue specimens will be required.
RNU43, RNU44 and RNU48 were recommended by manufacturers, because of their stability across multiple normal tissue types (Wong et al, 2007), rather than their stability between normal compared with tumour tissue, or different grades of tumour.
In addition, we developed a web interface titled 'LncRNA expression viewer' to facilitate investigators to graphically view the expression dynamics of genes across multiple human normal tissues.
The findings for calcium were similar to those for vitamin D. These findings indicate that supplemental vitamin D3 or calcium can favorably modulate multiple normal colon tissue and circulating hypothesis-based biomarkers of risk for colorectal neoplasms in sporadic colorectal adenoma patients.
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