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The B. neritina mitochondrial genome possesses 27 multiple noncoding regions.
Such multiple noncoding regions are rare in metazoan mitochondrial genomes.
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Interestingly, we found 27 multiplied noncoding regions (NC1-27).
It is well known that intergenomic phylogenetic footprinting is an effective method for the discovery of regulatory elements in a set of orthologous noncoding regions from multiple species [ 18- 22].
The wild-type and mutant bacterioopsin genes were cloned into pGEMHE, providing a multiple cloning site surrounded by the noncoding regions of β-globin from Xenopus laevis.
This identity encompasses noncoding regions as well as multiple mobilization sequences, offering not only evidence of lateral exchange but also a mechanism by which antibiotic resistance disseminates.
We also identified highly conserved elements within noncoding regions using whole-genome multiple alignments and gene expression data.
Ultraconserved regions [ 21] are noncoding regions > 200 bp perfectly conserved across multiple species.
In another study, comparison of PDXs at different time points detected multiple single nucleotide variants, but these mostly localized within the noncoding regions, suggesting that they arose over time during passaging [ 49].
In multiple cases where an ortholog was absent, we searched unsuccessfully for "missing" genes in noncoding regions of the assembly and unplaced reads.
In addition Single Nucleotide Polymorphisms (SNPs) in both the coding and noncoding regions of expressed genes can be identified by comparing the sequences of multiple matching reads with reference sequences [ 8].
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