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Seven cancer cell lines and one normal genomic DNA sample were studied with multiple mutations and polymorphisms identified among the 10 genes.
EMS induced mutagenesis is known to induce multiple mutations and this is one of the reasons as to why DNA fingerprinting is recommended apriori to their genetic characterization (Wu et al. 2005; Lima et al. 2015).
Also, the presence of multiple mutations and point deletions may alter the efficiency of the assay, possibly the reason for low performance in TP53 assays [16], [43].
These data will lead further investigations to explore the associations between multiple mutations and clinical outcomes.
In addition, this method can be adapted for simultanous detection of multiple mutations and quantitation.
The Lac+ papillae carry multiple mutations and have been attributed to stress-induced mutagenesis directed to sites that improve growth.
Similar(28)
Thirdly, multiple mutation and crossover operations based on the guided agent are employed to explore more effective solutions.
The explanation for the rare origins is almost certainly because complete resistance with high viability involves multiple mutations (Hastings and Watkins 2006), and so requires a highly unlikely series of mutational events to occur simultaneously.
Our results do however strongly resemble recent results with yeast populations in that multiple mutations arise and move synchronously through the population as mutational "cohorts" (Lang et al. 2013).
Furthermore, some researchers argue that CGVs also promote the acquisition of novel traits because of their ability to accumulate and release multiple mutations in individuals and populations [ 62, 63].
Since the reaction conditions are supposed to avoid a full digestion of the PCR products (and therefore not every mismatch is recognized in every strand), multiple mutations per fragment and their approximate position (± 10 bp) can be detected.
More suggestions(15)
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