Characterization of the effects of multiple mutants within the positively charged base region of the cysteine-rich loop demonstrated a sequential decrease in NF449 and suramin sensitivity.
However, the changes in ribosome profiles and rRNA structures in multiple mutants suggest the intriguing possibility that they may each contribute to refining the structure and function of the translational apparatus.
Multiple mutants of the CASP family display interrupted Casparian strips in the beginning, but rather quickly deposit more cell wall material in a delocalized fashion, eventually sealing the apoplast.
Sonntag, I., Schwarz, H., Hirota, Y. & Henning, U. Cell envelope and shape of Escherichia coli: multiple mutants missing the outer membrane lipoprotein and other major outer membrane proteins.
This yielded multiple mutants in the Fgf/Erk and GSK3/Tcf3 modules known to drive differentiation and in epigenetic modifiers implicated in lineage commitment.
This group analysed single and multiple mutants of the eight ACS genes in Arabidopsis using a combination of T-DNA insertion alleles and amiRNA technology.
We performed mono-infections because co-infection with multiple mutants can obscure the granuloma phenotype, owing to the wild-type helper effect.
The fact that random mutagenesis screens in zebrafish have yielded multiple mutants that disrupt the transport machinery suggests that at least some of the birth defects of unknown nature are due to abnormal protein transport (Neuhauss et al., 1996).
By contrast, the multiple mutants were up to 8-fold less efficient than the recombinant wild-type dalcochinase, and displayed primarily antagonistic interactions between these residues.
As a consequence, this vector will be a highly valuable means for the rapid generation of single or multiple mutants remaining sensitive to the most reliable selection markers Blasticidin S and neomycin.
