Sentence examples for multiple mutants of from inspiring English sources
These observations underscore the importance of exploring collateral networks in multiple mutants of different clinical strain backgrounds and with different resistance mechanisms to assess their potential clinical application.
Two multiple mutants of a psychrophilic α-amylase were produced, bearing five mutations (each introducing additional weak interactions found in pig pancreatic α-amylase) with or without an extra disulfide bond specific to warm-blooded animals.
The designed multiple mutants of LCI are unable to fold correctly, displaying a highly unstructured conformation and a very low inhibitory capability, which indicates the importance of disulfide bonds in LCI for both correct folding and achievement of a functional structure.
For this study, we generated multiple mutants of STBD1.
Multiple mutants of CTNNBL1 were also generated that carried amino acid substitutions in the putative NLS recognition groove (Fig. 4A).
It remains to be further investigated if multiple mutants of AAV-DJ could lead to enhancement in in vitro gene delivery.
As demonstrated by Aslanidi et al., a multiple mutant of AAV2 led to ~24-fold higher efficiency in in vitro gene transduction, which was mainly because the combined mutants had better ability for escaping ubiquitination then resulting in more nuclear translocation and more transgene expression [ 30].
Overall, using both a SA biosynthesis mutant and multiple mutants for regulators of SA accumulation, SA signaling and defense signaling, give support for the crucial role of SA as a regulator of cell death.
Other multiple mutants, all of which contained the K227Q mutation, formed dramatically reduced levels of undesired ligation products.
The same advantages apply for the construction of multiple mutants by crossing of strains with different gene deletions, which is now possible with considerably less hands-on time and minimal screening effort compared to a transformation approach.
Partial functional redundancy, which can be expected for some plant PLD isoforms, could be coped with by generation of multiple mutants, accompanied by monitoring of expression of individual genes upon various circumstances and by experimental analysis of promoters.
