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This is particularly useful when the genetic backgrounds of multiple mutants need to be maintained in a pure background.
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The multiple mutants showed denaturation temperatures up to 27 deg.
There, the pollen grains from tir1 and afb1afb2afb3 multiple mutants age prematurely (Cecchetti et al. 2008).
Using sequence- and structure-based approaches, 48 CLF multiple mutants were engineered and analyzed.
To evaluate the mechanisms regulating seed length, observation of microtubule arrangement and analysis of double mutants among srs1, srs3, Srs5, and various BR mutants need to be performed.
Double and triple mutants demonstrated some additive or synergistic effects, which were only inactivated by higher concentration H2O2, whereas multiple mutants A77E/A79S/I81L/M265L, A77E/A79S/I81L/M247L/M265L, and A77E/A79S/I81L/S168A did not.
This limited collection of mutant PAI-1 preparations contained multiple mutants defective in binding to three out of four tested anti-PAI-1 monoclonantibodiesies.
We next made crosses between ap2c1/ap2c2 with ap2c4/ap2c3 double mutants to obtain other combinations of multiple mutants.
For this study, we generated multiple mutants of STBD1.
In addition to previously described mutants [ 11, 12], we obtained multiple mutants by genetic crosses.
The double mutants or multiple mutants were derived from genetic crosses of the parental mutants (or transgenic lines).
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