Exact(2)
In principle, this can be accomplished through a combination of forward and reverse genetics and by constructing multiple mutants for redundant genes predicted to encode proteins with overlapping functions.
Overall, using both a SA biosynthesis mutant and multiple mutants for regulators of SA accumulation, SA signaling and defense signaling, give support for the crucial role of SA as a regulator of cell death.
Similar(58)
For this study, we generated multiple mutants of STBD1.
Comparison of multiple mutants provided evidence for the role of specific proteins from the Mtr respiratory pathway in this process.
Simultaneous survivals were then conducted for multiple mutants, in parallel with their isogenic wild-type controls.
The cost of HTS is continuously decreasing and by using multiplexing techniques like barcoding, a single Illumina sequencing lane can generate sufficient sequence at considerable depth for multiple mutants.
A total of 71 mutations were identified by TILLING, providing multiple mutant alleles for every gene tested.
A region of approximately 1500 bp from each gene was examined and a total of 71 novel mutations were identified by TILLING, thus providing multiple mutant alleles for each gene (Table 1).
The STM technique allows for the simultaneous screening of multiple mutants in mixed populations by detecting unique DNA tags.
The calculated stability score shows good correlation with the measured thermal stability for 76 single point mutations and 22 multiple mutants in β1AR.
The multiple mutants showed denaturation temperatures up to 27 deg.
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