Here, we used homologous recombination to engineer multiple mutants, and directly show that both ChoD and HsdD are dispensable for cholesterol degradation in fast-growing Mycobacterium smegmatis mc2155 and slow-growing Mycobacterium tuberculosis H37Rv strains.
Nevertheless, the data from multiple mutants and treatments allowed us to explore the suitability of direct sequencing as a miRNA profiling tool.
Our theory provides a unique explanation linking the molecular biology to the root hair phenotypes of multiple mutants and transgenic lines, including OX-ROP, CA-rop, aux1, axr3, tip1, eto1, etr1, and the triple mutant aux1 ein2 gnomeb.
It can be subdivided into two main approaches: either the network reconstruction is based on metabolite level correlation of multiple mutant and wild type samples, or on data from only one sample by using information of common reactions or similarity between metabolites.
Vast information on zebrafish development, molecular biology and genetics, successful large-scale mutagenesis programs and the availability of multiple mutant and transgenic phenotypes, make zebrafish uniquely suitable for studying genetic and early developmental factors that affect the aging process in vertebrates.
Since S32 substitution decreased the inclusion formation of multiple mutants including G93A and G85R, and as the obligate misfolded monomer G85R is a promiscuous acceptor of seed-induced SOD1 aggregation11,34, we focused our quantitative time-lapse cell-based aggregation studies on the pathogenic G85R mutant.
Using sequence- and structure-based approaches, 48 CLF multiple mutants were engineered and analyzed.
Overall, using both a SA biosynthesis mutant and multiple mutants for regulators of SA accumulation, SA signaling and defense signaling, give support for the crucial role of SA as a regulator of cell death.
Double and triple mutants demonstrated some additive or synergistic effects, which were only inactivated by higher concentration H2O2, whereas multiple mutants A77E/A79S/I81L/M265L, A77E/A79S/I81L/M247L/M265L, and A77E/A79S/I81L/S168A did not.
Multiple mutants were tested and only the variant present in the Pcsk5 gene was present in all of the affected mice.
Two founders, 36 and 40, contained multiple mutant alleles and were mosaics, as we reported previously on other targets (Carbery et al., 2010; Cui, 2011).
