The multiple mutants showed denaturation temperatures up to 27 deg.
There, the pollen grains from tir1 and afb1afb2afb3 multiple mutants age prematurely (Cecchetti et al. 2008).
Using sequence- and structure-based approaches, 48 CLF multiple mutants were engineered and analyzed.
Both multiple mutants display large modifications of stability and activity arising from synergic effects in comparison with single mutations.
By contrast, the multiple mutants were up to 8-fold less efficient than the recombinant wild-type dalcochinase, and displayed primarily antagonistic interactions between these residues.
This system will be useful for simple construction of unmarked mutations or repeated use of the same antibiotic marker to generate multiple mutants.
This limited collection of mutant PAI-1 preparations contained multiple mutants defective in binding to three out of four tested anti-PAI-1 monoclonantibodiesies.
The calculated stability score shows good correlation with the measured thermal stability for 76 single point mutations and 22 multiple mutants in β1AR.
In this study, we investigated the contribution of each particular disulfide to the folding, stability and function of LCI by constructing a series of single and multiple mutants lacking one to four disulfide bonds.
Here, we used homologous recombination to engineer multiple mutants, and directly show that both ChoD and HsdD are dispensable for cholesterol degradation in fast-growing Mycobacterium smegmatis mc2155 and slow-growing Mycobacterium tuberculosis H37Rv strains.
Double and triple mutants demonstrated some additive or synergistic effects, which were only inactivated by higher concentration H2O2, whereas multiple mutants A77E/A79S/I81L/M265L, A77E/A79S/I81L/M247L/M265L, and A77E/A79S/I81L/S168A did not.
