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In agreement with our observation of differential mt protein abundance (Fig. 5C), Zid and colleagues demonstrated preferential translation of mt proteins after CR in Drosophila (Zid et al., 2009) and CR has also been shown to increase mt gene expression and respiratory capacity in multiple murine tissues (Dani et al., 2010; Cerqueira et al., 2012).
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To understand role of BbCRASP-2 in B. burgdorferi infectivity, we first assessed the transcript levels of BbCRASP-2 in multiple murine tissue locations where B. burgdorferi persists during infection, and in various stages of infected ticks.
A western blot using various human and murine tissues exhibited multiple bands compatible with the theoretical molecular masses of the main isoforms of ZFAT (139, 137 and 94 kDa for proteins Q9P243 1, -2 and -3, respectively, and 128 kDa for another isoform), although some slight differences in isoform expression can be observed between tissues and species (Fig. 5D).
Our own expression analyses of AnxA1 in breast cancer tissue arrays from 141 patients—and in multiple murine and human tumour cell lines and tumour types clearly establish that AnxA1 is downregulated during late stage of tumour progression and metastasis (Figs 1D G; S1andnd B).
Multiple murine models have proven useful in studying the natural history of neovessel development in the tissue engineering of vascular grafts.
CAMGAP1 mRNA is widely distributed in murine tissues.
Murine tissues express three mRNA variants, encoded by two genes on chromosomes 2 and 3.
However, the full expression pattern of human VMD2 within murine tissues is unknown.
Other murine tissues were similarly processed.
SOCS6 mRNA was expressed ubiquitously in murine tissues [ 54].
Murine tissues were collected from six-month-old C57BL/6 mice.
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