The most common defect observed was multiple mitotic spindle poles rather than the expected bipolar structure.
Tim depletion caused multiple mitotic defects, including the loss of sister-chromatid cohesion, loss of mitotic spindle architecture, and a failure to exit mitosis.
Disruption of Notch signaling results in continued proliferation and expression of mitotic markers and immature cell-fate markers in follicle cells beyond stage 6 [27].
Apparently, the multiple mitotic functions of SUMO carried out by the single SUMO isoform in Drosophila have been divided among multiple SUMO isoforms in vertebrates.
Soon after their formation, trimera-derived tetraploids undergo mitotic missegregation events involving multiple mitotic spindles.
It seems plausible that the combined action of multiple mitotic, Cdk1-regulated, factors fine-tune mitotic exit.
A strong anisocytosis, numerous cells with multiple nuclei, anisokaryosis, multiple mitotic figures, to some extent atypical mitotic figures, and a varying nucleus: plasma ratio could be detected.
For example, polo-like kinase 1 (Plk1) is a well-studied mitotic kinase whose inactivation leads to multiple mitotic defects and cell death.
The downregulation of the DAPK/pS308 signal in cells trapped in mitosis with stable levels of mitotic markers (Plk1, pH3) confirmed that BI2536 inhibits DAPK activity in mitotic cells and downregulation of the DAPK/pS308 is not a result of mitotic exit.
In addition to multiple mitotic functions, several recent reports have established a link between Plk1 and DNA replication [ 10- 12].
