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Taken together, these data indicate that Tim is required, directly or indirectly, for multiple mitotic events, including chromosome condensation, sister chromatid cohesion, bipolar microtubule organization, and microtubule assembly on condensed chromatin.
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This requires complex chromosomal rearrangements indicating that multiple mitotic recombination events occur frequently in soft tissue sarcoma tumorigenesis.
Soon after their formation, trimera-derived tetraploids undergo mitotic missegregation events involving multiple mitotic spindles.
In any event, inspection of the consensus sequences of the phosphorylation sites (Supplementary Figure 2B) suggests that multiple mitotic kinases contribute to control Ska3 function.
Mitotic events were scored by time-lapse video microscopy and DNA staining.
Mitotic events of control, cells were scored by their morphological change (from flat to round-up).
In the proposed method, three phases are defined for representation of mitotic events.
The most common defect observed was multiple mitotic spindle poles rather than the expected bipolar structure.
For H3P quantifications, all the mitotic events were determined by counting nuclei labeled with the anti-H3P antibody.
We also found that the mitotic events after RNAi knockdown of two genes (chd4 and mex3-1) changedifferentlyly (Fig. 1C).
The conserved kinase AIR-2/Aurora B is required for normal execution of all these important mitotic events in Caenorhabditis elegans.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com