Sentence examples for multiple mice were from inspiring English sources

To evaluate Foxp3 maintenance or granzyme B induction in vitro, CD4+CD25+ regulatory T cells from multiple mice were pooled and sorted, and cultured in multiple wells (2 4×104 cells/well) in complete media for 18 or 40 hrs with irradiated T cell-depleted spleen cells (APC; 1×105) and anti-CD3 antibody (0.5 µg/ml).

By setting the MPI threshold to 0.95, vocal signals were not assigned to a mouse when multiple mice were in close proximity to the estimated source.

Multiple mice were subsequently infected with either single-sex or mixed-sex cercariae and the worms harvested 51 days post-infection.

In our experiment multiple mice were tested under each experimental condition, and the unpredictably of mouse production and maintenance led to sporadic imbalance of drug vs. placebo mice per diallel category.

Cells from multiple mice were pooled and incubated with labelled antibodies to epithelial cell adhesion molecule (EpCAM-APC; eBiosciences) diluted 1 50 in 2.5% FBS in PBS for 30 min on ice, then sorted using a FACS Aria flow cytometer (BD Bioscences).

The issue of animal handling when imaging multiple mice is further discussed in section entitled "Issues related to animal handling".

Typical flow plots are shown in (B ) and cell frequencies and absolute cell numbers from multiple mice are pooled in (C ) and (D ).

(1) The most confident calls were derived from multiple mice and were fully concordant.

The majority of 'present' and 'not detected' calls (12,115, 73.3% of all data) were derived from multiple mice and were fully concordant within our data set.

We separated the single-sex adult worms in multiple pools (each one containing >500 worms which were originated from multiple mice) which were frozen at -80°C until further processing of the samples.

In the present study, the ratio of transgenic integration was as high as 54%, and the interfering fragment expression in multiple F1 mice was shown to be effective.