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GR_4 was focused on a targeted gene and considered multiple miRNAs targeting the specific gene when calculating the inter-relationship.
Although a single gene can elicit an effect or phenotypic disturbance, multiple miRNAs targeting a common gene may be more efficient.
Accordingly, there should be multiple miRNAs targeting those genes involved in tumorigenesis.
Multiple miRNAs targeting the same mRNA may function together during gene expression process.
So we took multiple miRNAs targeting one RNA into account in our study.
The large number of hits reflects the well-known fact that there are multiple miRNAs targeting an individual gene.
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We also found that multiple miRNAs target the same mRNA (Table S13).
It is well known that multiple miRNAs target the same gene, and it is not the one miRNA one mRNA connection, it is the combination of multiple miRNAs that target the same gene should be considered (Peter, 2010).
While it has been shown that miRNA cluster may function through the cooperation between individual miRNAs to regulate the specific signaling pathway, a recent study is probably the only defined example that experimentally confirms that multiple miRNAs target the same gene.
Conversely, each mRNA may harbor multiple miRNA targeting sites (Ala et al., 2013).
This can be achieved by enhancing mRNA degradation through an additive effect from multiple miRNA targeting.
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