In fact, many known imprinted genes that we failed to identify on the basis of these criteria did show nominally significant (P < 0.05 without correction for multiple comparisons) DNA methylation differences between diandric and digynic triploids (Table S3 in Additional file 2).
When we evaluated the individual CpG loci, we identified 190 CpG loci with significant methylation differences after correction for multiple testing.
SAM then serves as a source of methyl groups for multiple methylation reactions, including the methylation of DNA.
All glycans have multiple methylation sites and, owing to the multiplication of the mass-shift, mass differences for the discrimination of differentially labeled glycan pairs to be analyzed using high-resolution mass spectrometers (e.g., Fourier transform MS) are obtained.
Association between severe caloric restriction,, persistent DNA methylation differences of a similar magnitude at multiple epigenetic targets including IGF2 and MEG3,, and poor health outcomes, including LBW, have been previously reported.
The major proportional components of peripheral blood cells are similar between all three species [ 32- 34]; therefore, methylation differences affecting the major cell type fractions or multiple subtypes will be detected.
Statistical analysis to determine significance of clade methylation differences was performed using SPSS IBMM, New York, NY) by ANOVA with multiple comparisons Tukey adjustment.
As an example, Rakyan and colleagues identify small disease-associated methylation differences of 1.3 to 6.6%, which compare well with other multiple referenced studies in complex disease and our data [ 20].
However, it is also possible that subtle (less than two-fold) changes in gene expression caused by DNA methylation differences could have important consequences, particularly if those genes are of major or multiple effects.
However, although the mechanism by which such differences might act is unclear, methylation differences of this magnitude are associated with significant differences in transcript level at multiple genes (Table four and Figures two and three in [ 1]; Tables one and five in [ 4]).
