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For human tissues, expression values were averaged to yield a single expression value when multiple metastatic nodes were collected on a given individual.
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Patients received post-operative radiation therapy in any of 3 situations: 1) metastatic cancer extending beyond the lymph node capsule 2) multiple metastatic lymph nodes 3) positive surgical margins.
The presence of multiple metastatic lymph nodes (LNs) is presumed to be a worse prognostic sign [ 3].
Sixty-five patients with multiple metastatic lymph nodes in the surgical specimen received docetaxel or CDDP plus 5-FU postoperatively (Ando et al, 2003).
Mediastinoscopy was not a prerequisite if there were multiple metastatic lymph nodes and at least one of them was more than 1.5 cm in diameter in a computed tomography of the chest.
Only a few patients who showed multiple metastatic lymph nodes in the surgical specimen received a regimen of docetaxel or CDDP plus 5-FU after operation (Ando et al, 2003).
About 10 months later, she developed multiple metastatic lymph node lesions in the abdomen.
Computed Tomography (CT) additionally revealed huge mediastinal lymphatic masses and significantly enlarged lymph nodes in the spleen hilus and left kidney as well as ascites and multiple metastatic nodules within peritoneum (Fig. 1).
One of the patients was deleted from the study as the histological evaluation revealed multiple metastatic and converging lymph node packages.
The present method could become a valuable tool to quantify dissociated responses on multiple tumour sites, e.g. primary tumour and metastatic nodes, provided that all the regions of interest are selected.
Taking the advantage of red fluorescent signaling, multiple metastatic foci in liver, lung as well as lymph nodes of posterior peritoneum were easy to be visualized in nude mice as early as 2 weeks after orthotopic transplantation.
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