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Exact(7)
As exon 5 contained multiple melting domains, we divided the exon into two amplicons for further screening.
In rare cases, a specific amplification reaction showed a so-called 'shoulder peak' (29) which occurs within amplicons containing multiple melting domains with varying Guanine-Cytosine (GC) contents.
As expected, since the PCR fragments had an average size of 600 bp, HRMA showed a wide range of melt profiles often containing multiple melting domains per fragment representing differences in nucleotide sequence.
We hypothesized that the multiple melting domains were masking the presence of the mutation in BT-20.
PCR amplicon with several melting peaks would be showing the presence of multiple melting domains and may produce complex melting profile that maybe hard to interpret.
Whereas HRM is able to detect all heterozygous mutations and some homozygous mutations within amplicons with complex melting domains, DHPLC sensitivity and specificity can be challenged within multiple melting domains.
Similar(53)
These include the bubbles and helical regions, bubble nucleation sites, cooperative melting domains, melting fork junctions, breathers, sites of high or low stability, and SIDD sites.
In contrast to the N-terminal half, the LRR domain is stable and compact and forms a cooperatively melting domain.
All amplicons were chosen so that they contained a single melting domain using the Poland program [ 11].
PHBVHHx exhibited also different multiple melting behaviors from PHBHHx.
It is this multiple distribution of lamellar thicknesses that causes multiple melting behaviour.
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