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Tumor specificity of antibody cultures was evaluated by comparing binding of antibodies from these cultures against multiple melanoma cells (A-375, SK-MEL-2, WM-115) versus normal cells (Figure 5, A).
Both can be induced towards a tumor promoting state in the tumor microenvironment: mDCs contribute to the survival of multiple melanoma cells [ 121].
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The expression of ROR1 is detected in multiple melanoma cell lines, as assessed by RT-PCR, Western blot, and flow cytometry.
However, we report that reduction of Mcl-1 expression alone is not enough to significantly reduce survival in multiple melanoma cell lines.
Rounded morphology was induced by transfection of miR-200c in multiple melanoma cell lines when plated on top of a thick deformable layer of collagen (Fig. S2).
Here we present evidence that the combination of ABT-737 and Mcl-1 knockdown by DsiRNA efficiently induces cell death in multiple melanoma cell lines well over the effect seen with the individual treatments.
Quantification of the proportions of cells exhibiting different modes of invasion inside the collagen layer reveals that miR-200c induces up to a 2-fold increase in the proportion of invaded round cells in multiple melanoma cell lines (Fig. 1C, because mock and scrambled results were highly similar across experiments, only scrambled control is presented for other experimental data).
Wnt3a enhances TRAIL-dependent apoptosis in multiple melanoma cell lines [ 53].
We previously found TGFβ to decrease cell viability in multiple melanoma cell lines, isolated from different patients [ 12].
These results indicate that WLS is required for endogenous WNT/β-catenin signalling in multiple melanoma cell lines.
Together, these data suggest that WLS expression may be regulated by the activation of WNT/β-catenin signalling in multiple melanoma cell lines.
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