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Of the five extinctions, three were from the single and two from the multiple mating treatments.
Sample size (N), Mean and Standard Error of the Mean for single and multiple mating treatments at F0, F1 and F2, for each component of fitness.
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The greater fecundity in the multiple mating treatment was driven by the over-production of viable sons (> 87%).
At three months old, F0 females and F0 males were haphazardly allocated to either a single or a multiple mating treatment.
F0 males allocated to the multiple mating treatment were not rotated among different replicates (i.e., each group of four males was only used in one replicate).
Therefore, to obtain a total of 40 broods for each treatment, 73 and 78 femalesles were mated for the single and multiple mating treatment, respectively (Table 1, Additional file 1: Supporting information 1).
On the second day, in the single mating treatment the same male was introduced to the same female as in day 1, whereas in the multiple mating treatment a novel male was introduced to the female.
Although we cannot rule out the effect of "ideal laboratory conditions" in alleviating the costs associated with multiple mating, the fact that we did not find any effect of multiple mating in costs that are measurable under laboratory conditions and in any of the life history traits studied indicates the costs were negligible in the multiple mating treatment.
Sex ratio (SR-male/female) of F1s was not significantly different from a 1 1 in the single mating treatment (SR = 0.92; P = 0.71, Additional file 1: Supporting Information 1), but was male-biased in the multiple mating treatment (SR = 1.44; P = 0.03, Additional file 1: Supporting Information 1).
Mesocosm populations from the 'multiple' mating treatment showed significantly greater between-fish complementarity in colour patterns (F1,30 = 6.432; p = 0.017), thus suggesting that multiple mating had indeed led to multiple paternity in this treatment.
If F0 females in the singly mated treatment retained brood production, then this can be seen as an advantage of multiple mating over single mating.
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