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The reduced size of the ejaculate of sex-ratio males, in addition to direct consequences of their fertility, affects the behaviour of females under multiple mating conditions through their sexual learning capability.
While under multiple mating conditions the net result is at disadvantage of XSR over XST in both studies, our data indicate that when the matings are rare the outcome of the competition could differ, depending on the distortion strength.
The sperm release phenomenon (i.e. dumping, [ 7]), which represents an additional load to SR males in multiple mating conditions, can be linked either to first male or second male characteristics or interactions with the female reproductive tract (i.e. female cryptic choice; [ 6]) or various combinations of those traits.
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As our study shows, under benign conditions, multiple mating brings a ~1.5 fold increase in female fecundity.
However, sufficient multiple mating should reverse this condition; matrigenes should be more altruistic and patrigenes more selfish.
We found that female fertility was positively correlated with the eyespan of their mates under conditions of multiple mating.
Columns and rows as in Table 2. Multiple mating does not change these conditions.
We chose to assess male fertility under conditions of multiple mating, which are typical in this species [ 43, 44], because we had previously shown that there was no fertility difference between females mated once to large or small eyespan males [ 7].
Multiple mating may depend upon females' condition, but we found no evidence that female inbreeding status influenced that rate of multiple paternity, regardless of the males' genetic similarity.
Since the frequency of multiple mating is similar in laboratory and wild conditions [ 52], the above costs are therefore predicted to be comparable (although the prevalence of disease is expected to be greater in the wild).
The condition reverses with sufficient multiple mating by the queen, because then the paternal genes are rarely related.
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