The volume-of-fluid model is based on a multiple-marker method with a smoothed surface tension calculation, and a thin-film model is derived to simulate film drainage using a Reynolds equation approach.
In particular, given the single-marker test statistics, a multiple-marker method was developed to predict test statistics for markers for which a sire was homozygous (or at any other location on the chromosome).
Six half-sibling families with a total of 285 sons organized according to a granddaughter design were analyzed by a multiple marker regression method.
Our newly developed multiple marker (MM) method (Lindstrom and Thornell 2009) was used to compare staining for MyoD, myogenin, c-Met and Dlk1 with staining for NCAM or Pax7, which are the reference markers of SCs.
A genome-wide correction to correct for multiple marker testing was applied (Bonferroni method: p < 6.4 × 10−8 calculated from nominal p value of 0.05/total number of markers as 782,838).
It differs from previous methods in that it uses information from both multiple markers and multiple methods of analysis.
A multiple marker Coupled Level-Set/Volume-of-Fluid (CLSVOF) method for droplet-laden flows has been extended with a physical model to accommodate coalescence and breakup of the droplets.
The final method of analysis is a multiple marker real-time RT PCR assay.
Current test methods require additional serum for each marker, although alternatively methods were recently introduced that allow multiple marker testing in small volumes in the range of finger prick serum or DBS volumes.
Among the existing methods, some consider single markers linked to the selected locus (e.g., Slatkin 2001; Kim and Stephan 2002), whereas more sophisticated methods gain information by exploiting the haplotype structure of multiple marker loci.
Among all available mapping methods, the multiple-QTL approach is particularly suitable for complex trait analysis since it uses simultaneously multiple marker intervals with possible inclusion of epistasis terms in QTL mapping model [ 23].
