The remaining part of the gradient, which included mRNAs associated with one or more ribosomes, was fractionated to multiple fractions of equal volume.
Commonly enriched GO terms among mRNAs associated with selected proteins were retrieved with the GO Term Finder that uses a hypergeometric distribution with Multiple Hypothesis Correction to calculate p-values (SGD; www.yeastgenome.org).org
Gene Ontology (GO) term enrichment for the mRNAs associated with each candidate RBP was calculated comparing these mRNA targets to all the genes that had green signal above background on the microarray using the hypergeometric test (Figure 3), correcting for multiple hypothesis testing [43].
Moreover, CT-activated IRE1 degrades mRNA associated with the ER membranes, a mechanism termed regulated IRE1-dependent decay (RIDD).
The enrichment of low p-values among the miRNA-mRNA relationships suggests that our study is capturing some true signals and, of these, miRNAs tend to be associated with multiple mRNAs (as perhaps expected from the fact that miRNAs are known to target at least a third of all genes in the genome [ 18]).
In most of these cases, the periodic behavior observed at the macroscopic scale is associated with periodic changes in the level of multiple mRNAs.
Individual miRNAs may target multiple mRNAs.
By splicing precursor (pre-) mRNAs in different ways, multiple mRNAs and proteins can be generated from a single gene.
A miRNA can target multiple mRNAs, and, conversely, an mRNA can be targeted by multiple miRNAs.
Individual miRNA may target multiple mRNAs, and individual mRNA may contain sequences complementary to multiple miRNA family members [ 13, 14].
