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Similar effects of forced differentiation on ACSVL3 expression levels were seen in multiple low passage primary GBM neurosphere isolates.
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Of note in this report is that although mouse IPSCs at low
Mammalian expression vectors for HPV16 E6 and HPV16 E7 were generated by subcloning HPV16 E6 cDNA or E7 cDNA, freshly cloned from low passage SiHa cells, into the multiple cloning site of pcDNA3.1.
In contrast, the low passage B. burgdorferi B31 clone B31-5A18NP1 [14] exhibits a much more complex surface, where multiple abundant proteins such as Osp's might at least partially mask the expressed Vsp proteins and therefore lead to a somewhat attenuated change in phenotype.
Micropatterning was as effective as TGFβ1 in up-regulating SM-MHC at low passage; however, micropatterning limited VSMC response to TGFβ1 at both low and high passage.
Low passage hESCs (H9, WiCell) were used.
In all experiments with MEFs, low passage (less than 3 passages) MEFs were used.
Each cell line high passage miRNA array was normalized to its corresponding low passage miRNA array.
All analyzed strains represent low passage (1 2 passages) isolates that are as close to the "wild" type viruses as possible.
Low passage frozen stocks were prepared and early passage cells were used in the experiments (less than passage 10).
The experiments were performed at passages 8 or 14 (low passage samples) or 25 (high passage samples).
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