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This method allows multiple low abundance proteins to be quantified simultaneously with excellent accuracy and reproducibility [ 67].
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The combination of a unique Farber disease model with on-tissue enzymatic and CID structural confirmation facilitated development of a novel and comprehensive MALDI-IMS workflow to identify and visualize multiple low-abundance sphingolipid metabolites directly in tissue sections.
This PEA-based approach provides a platform for accurate quantification of multiple (96) low abundance oncoproteins from biological samples.
Apparently, accessibility is facilitated in a highly variable region of the S. solfataricus genome with multiple IS elements and low abundance of essential genes.
Since conventional serotyping methods are biased towards detection of the most abundant serotype, the VTs present at low abundance in multiple carriage events may not have been detected in these samples, which could have resulted in poor estimation of the vaccine coverage.
Amino acid multiple alignments of the salivary gland low abundance proteins.
On the other hand in the brain, in which CLs are present at low abundance and with multiple isoforms, imaging has not been possible.
This read assignment uncertainty will affect the accuracy of isoform expression quantification and introduce noise, especially for low abundance genes with multiple isoforms.
Multiple different dilutions were tested to ensure that low abundance saccharides were detectable.
The de novo assembly of a transcriptome presents multiple challenges including computational requirements and accurate assembly of low abundance transcripts.
In addition, low abundance small RNA species that can target multiple RPP5 locus R genes exist in wild-type plants, and transgenic over-expression of SNC1 can induce the cosuppression of these paralogous R genes [ 6].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com