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Recombination events at multiple loci in individual cells are described here.
The phenotypic data of these preliminary analyses, shown in Figure 3A and B, suggested the involvement of multiple loci in panicle blast resistance, similar to that in Figure 3C.
By delivering CRISPR/CRISPR-associated protein 9 (Cas9) ribonucleoprotein (RNP) complexes under conditions of rapid B cell expansion, we achieved site-specific gene disruption at multiple loci in primary human B cells (with editing rates of up to 94%).
In this work, we consider a complementary approach that involves the characterization and measurement of the similarity and dissimilarity of the allelic composition of a set of individuals' diploid genomes at multiple loci in the regions of interest.
The most abundant small RNAs in M-MLV reverse transcriptase (copy numbers > 10000) are listed in Table 1 and Supplemental File 2. BLAST sequencing showed that most of the RNAs were fragments of rRNAs (16S and 23S), and we noted that many small RNA fragments have multiple loci in the E. coli genome.
The most recently developed of these tools, the system of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), consists of an RNA-guided endonuclease that can be repurposed to edit both genome and epigenome with high specificity, and facilitates the functional interrogation of multiple loci in parallel.
TSM may be able to evaluate overall association strength of multiple loci in association studies.
These data suggest a complex interplay between multiple loci in the regulation of GL, which will require further approaches to identify individual genes that regulate parturition.
Multiplex ligation-dependent probe amplification (MLPA) can simultaneously detect copy number changes of multiple loci in one simple PCR reaction, making it an attractive alternative to FISH.
In multilinear regression, target trait values can be modeled as a function of independent variable vector corresponding to the genotypes of multiple loci in candidate genetic regions.
Resequencing studies of unlinked, noncoding, multiple loci in ethnologically well-defined populations from Central Asia are needed to address this question in the context of Eurasian prehistory.
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