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In practice, AIL populations generated in the lab have generally been expanded in the final generation either by generating multiple litters from the same crosses (the UTHSC cross described below for example), or by retaining a larger number of animals in the penultimate generation and using this expanded set of animals to produce one litter per cross [6], [9].
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MMTV/Neu transgenic mice (FVB/N-Tg(MMTVneu 202 Mul/J) were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and bred to produce multiple litters for colony maintenance.
Extensive molecular and protein analysis of 26 different p63 Brdm2/Brdm2 embryos from multiple generations and multiple litters ranging from day E13 to P1 consistently revealed expression of aberrant truncated p63 variants, not confined to random epidermal patches but widespread, and associated with a transient phenotype consistent with residual functions of the targeted allele, as detailed below.
Female offspring of High (n = 15) and Low (n = 11) LG mothers from multiple litters were examined daily from day 22 to 35 of life to determine the day of vaginal opening.
Number of cells analyzed is stated in Figure 2H counted from 3 4 hearts per marker, each from multiple litters.
Animals were randomly selected from multiple litters; age selection was based on colony availability.
Subjects, in total 25, were 5 7 days old male and female Wistar rats, weighing 12.0 22.6 g, from multiple litters.
Newborn pups from multiple litters were pooled and then randomly distributed to exposure to room air (21% O2), 95% oxygen, or 70% oxygen for 14 days starting between 12 and 24 h after birth.
Mice from multiple litters (at least three) were used for each treatment group (n = 20 40; equally divided between genders).
The rats came from multiple litters.
Males from multiple litters were randomized to avoid placing littermates in the same experimental groups.
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