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A major barrier to applying these methods to HSCs/HPCs arises from the requirement that multiple lineage markers be used to enrich for the LIN− HSC/HPC compartment (Materials and Methods).
In contrast, control FF-hESCs that were maintained in DMEM (10% FBS) for 3 weeks without the chondrocyte co-culture expressed multiple lineage markers, indicating heterogeneous differentiation of the hESCs.
Adult murine HSCs can be prospectively enriched by their low expression of multiple lineage markers (LIN−) including CD127, and high expression of c-Kit (hereafter, Kit) and Sca-1, as well as several other markers [1].
Thus, cells in Chd4 −/− embryos continue to express multiple lineage markers even at the blastocyst stage, and fail to establish exclusive lineage-appropriate gene expression programmes.
The TDLU-like organoids form alveolar buds, express multiple lineage markers at correct positions and display contractility, which our assay reveals to be required for alveologenesis.
These two classes differ in the frequency of expression of Group 3 genes: Class II cells express these genes at a higher frequency than do Class I cells, with the consequence that they are much more likely to show simultaneous expression of multiple lineage markers (Fig. 5A).
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To characterize the properties of dissociated NPCs, expression levels of multiple neural lineage markers were examined by immunofluorescence staining.
While these approaches have identified multiple lineage restricted endodermal markers not all of the "hits" have been validated by further experimentation.
To determine whether mammosphere-cultured cells from pleural effusions taken from late-stage breast cancer patients could differentiate into multiple lineages, cells were placed in differentiating conditions and then stained for lineage markers.
Furthermore, hADSCs exhibited low expression of hematopoietic lineage markers CD45 (1.03%) and CD34 (2.88%).
Also, hAFSCs showed bright expression of mesenchymal lineage markers and immunoregulatory properties.
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