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(Update: my mistake, multiple libraries were already available).
Successive multiple libraries were used in the long march [ 10] and SubAssembly[ 11].
Poly(A+) RNA was isolated, size selected and multiple libraries were made in the lambda-ZAP Express vector by unidirectional cloning of cDNAs using the kits of Stratagene.
In order to get wide transcriptome coverage, multiple libraries were constructed from leaves, roots, shoots, flowers, ovaries and fruits of different citrus species under different stress or developmental conditions (Table 1).
Poly(A+ RNA was isolated, size selected, and multiple libraries were made in the Lambda-Express vector by unidirectional cloning of cDNAs using kits of Stratagene (Santa Clara, CA, USA).
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The ability to have multiple libraries is nice; splitting work and personal stuff would be my move, so that if a meteor crashed into TC HQ (or, more likely, I'm fired for insubordination), I could free up a couple gigs in one clean sweep.
The intent of the multiple libraries was to cover the maize genome thoroughly, as each library is expected to sample a somewhat different space.
To increase the number of TSSs/TTSs identified from directional RNA-seq libraries, a strategy of combining multiple libraries was adopted.
The ESTs that are contained in each contig are listed in the additional file 2. The distribution of ESTs across multiple libraries was assessed as a measure of uniqueness of gene concurrence across libraries.
A major advantage of EST sequencing from multiple libraries is that it increases the possibility of identifying genes that are putatively transcribed specifically within a certain tissue, during a particular developmental phase, or under some environmental conditions.
Another major advantage of sequencing ESTs from multiple libraries is the ability to identify genes that are putatively transcribed specifically within a certain tissue or during a particular developmental phase.
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