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SNPs appearing at low frequency (< 10%) in multiple libraries corresponding to different cells were removed because they probably represented sequencing artifacts.
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For D. melanogaster, we retrieved multiple libraries representing four embryonic data sets (0 1, 2 6, 6 10, and 12 24 h, corresponding to the phases of cleavage divisions, gastrulation and germband elongation, germband shortening, morphogenesis, and organogenesis, respectively), larval, and mixed-sex adult data sets (Ruby et al. 2007; Chung et al. 2008).
NOTE: Course lists may not be duplicated at multiple libraries!
It may, more likely, be multiple libraries of Alexandria.
(Update: my mistake, multiple libraries were already available).
This is particularly true when one wishes to select a complete library rather than to cherry pick a set of compounds from multiple libraries.
If your university has multiple libraries, stretch your legs and mobilize.
All sequences were derived from multiple libraries.
How does high coverage by a single library compare with the use of multiple libraries?
It contained 21400 clusters defined from EST libraries corresponding to different M. truncatula tissues.
In pooling of multiple libraries for running on a single lane, equal amount of library mass was determined by Qubit reading and Bioanalyzer for the 5 RNA libraries.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com