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Without any loss of generality, we can suppose the set of multiple labels of all bags be denoted as (mathbf{Y}= {mathbf{y}_1,mathbf{y}_2,ldots, mathbf{y}_N}), (mathbf{Y}in mathbb {R}^{ltimes N}).
Our approach using multiple labels of proteins in the same tissue section showed that human SCs are more heterogeneous than previously recognized.
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The combination scheme described above allows the output of multiple labels on each frame.
However, these techniques required multiple labeling of oligonucleotide substrates.
These techniques still require multiple labeling of oligonucleotide substrates, as well as advanced technology not readily available in most laboratories.
This broad signal may be because of the protein adsorption frequently observed in CE, or the multiple labeling of the F-BSA used in this work [ 29].
(11) To alleviate the issues of multiple labeling of analytes, we used the commercially available iTRAQ kit for quantitation via MALDI MS. The iTRAQ kit includes a set of four isobaric reagents that react with the amine groups of peptides.
To assign multiple labels to a given set of sequences, we employed the training method used for gene detection in Drosophila [29].
From each rat, selected sections were processed for (1) immunohistochemical labeling for VGLUT2; (2) detection of tracers; (3) multiple labeling for detection of the anterograde or retrograde tracers and immunohistochemical detection of VGAT or GAD65 and VGLUT2; (4) double labeling for detection of NeuN and VGLUT2; and (5) nonradioactive in situ hybridization using VGLUT2 cRNA as a probe.
The Meat Puppets are still soldiering on after 14 albums, multiple labels, bouts of rehab, and some mainstream exposure thanks to an appearance on Nirvana's MTV Unplugged show in 1993.
Table 10 Total number of the elements participating in COSMOROE relations in GWW, with and without multiple labels Elements No. of annotations No. of annotations (duplicates excluded) Utterances 347 223 Objects 728 140 Body movements 153 73 Acoustic events 125 49 Gestures 34 134
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