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All STs with multiple isolates were composed of isolates with the same serotype.
Multiple isolates were subcultured from one or both of the two different selective agar plates for each sample.
Furthermore, although decreasing viral fitness coincided with decreasing plasma VL for 4 of 6 subjects from whom multiple isolates were obtained, overall, viral fitness did not correlate with plasma VL for the 14 PULSE subjects investigated (Borderline statistical significance p = 0.051; Figure 5).
Multiple isolates were collected from 17 women.
Multiple isolates were associated with a significant increase in mortality.
One sequence type was found for 6 of 9 patients for whom multiple isolates were typed.
Similar(41)
There is a high level of cryptic diversity; of the 17 "species" for which multiple isolates are examined here only eight are monophyletic [ 17].
In the case where multiple isolates are linked to the same outbreak (e.g. outbreak 129 547 in Table 1), some pairs are established with isolates at rank ≤2.
Our data also suggests that, if multiple isolates are obtained from a suspected source population, there will likely be added value in generating WGS data on many or all of them rather than sequencing a representative isolate.
The co-occurrence of var types found in multiple isolates is significantly higher than for the null expectation under the assumption of linkage equilibrium among all var types (pv<0.01) (Artzy-Randrup and Stone, 2005 ) (the expected co-occurrence matrix conserves both the observed number of var types in each isolate and the observed frequency distribution of different var types (Chen et al., 2011 )).
During the study period, a total of 82 children with multiple MRSA isolates were identified.
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