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The selection criteria of these strains were based on the heterogeneity in their properties such as, geographic origin, time of isolation, levels of resistance to carbapenems, aminoglycosides and fluoroquinolones, thus excluding multiple isolates of the same strain from one locality.
Therefore, in this study we examined the variation in growth and morphology of multiple isolates of both species, isolated from two adjacent Australian reservoirs.
Sensitivity and repeatability were established, and specificity was verified by testing multiple isolates of EHV-5, as well as DNA from other equine herpesviruses.
The utility of PrimerSelect for designing primer pairs complementary to ORF sequences defined for multiple isolates of the human bacterial pathogens Helicobacter pylori and Staphylococcus aureus is also demonstrated.
Pan-genomic reverse vaccinology, with the comparison of sequence data from multiple isolates of the same species of a pathogen, increases the opportunity of the identification of novel vaccine candidates.
It is likely that many genes show protection effects against several pathogens and multiple isolates of one pathogen.
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The presence of multiple isolates for 17 of these novel species further supports their status as potential pathogens.
In addition, it was tested whether differences in FA patterns can also be found for groups at lower taxonomic rank, i.e. between species of the same genus or even among multiples isolates of the same species.
Studies on P.falciparum have also used the sequencing of multiple isolates to look at the fine-scale structure of genome evolution and population genetics [9] [11].
Due to the volume of isolates collected and the laborious nature of PFGE, only one isolate was analysed per sample type which may have under-estimated the genetic diversity of E. coli O157 H7 compared to analysis of multiple isolates [ 20], although analysis of a single isolate is generally indicative of the dominant strain [ 21, 22].
Whole genome re-sequencing of multiple isolates will determine the pattern of sequence polymorphisms [ 113] and structural variations [ 18] in pathogen populations and their association with pathogen aggressiveness and host range.
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