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Genome sequencing of multiple isolates allowed us to determine a genome-based phylogeny of E. faecium and to accurately quantify the substantial genomic diversity between strains in this species.
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A few genera were well represented with multiple species and isolates, allowing a comparison among species within a genus with multiple hosts.
Type cultures were used where available, as were multiple isolates.
All STs with multiple isolates were composed of isolates with the same serotype.
Multiple isolates were collected from 17 women.
Because TAR cloning produces multiple gene isolates, it allows the isolation of both parental alleles of the gene which can then be used for haplotype analysis [ 24].
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Thus, the use of quantification and Oct4ΔPE GFP to isolate germ cells at multiple timepoints allows uncovering of novel phenotypes relative to other methods [22], [26], [52].
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