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For m6A peak calling, the longest isoform was used if multiple isoforms were detected.
When multiple isoforms were present, one representative was selected randomly.
In cases where multiple isoforms were present, we chose the longest isoform.
The RPKM values for subunits of a protein and for multiple isoforms were summed.
When multiple isoforms were present, we removed all but one representative sequence based on the following criteria.
This technique retains the isoform that is most abundant across all clones, when multiple isoforms were present.
Similar(48)
These derivatives were investigated as inhibitors of the metalloenzyme carbonic anhydrase (CA, EC 4.2.1.1) of which multiple isoforms are known, and some appear to be involved in pain.
The observation that the synaptic vesicle proteins, all occurring in multiple isoforms, are differentially expressed with respect to each other indicates that up to 90 different vesicle types are possible.
Although these multiple isoforms are predicted as key in 5-HT2CR functhere there are no published methods to separately analyze expression of isoform-specific proteins; however, high-throughput sequencing provides the opportunity to evaluate expression of mRNA for all 5-HT2CR isoforms across phenotypes (Morabito et al, 2010; Zhu et al, 2012).
Results from the RT-PCR analysis of the GABA receptors and transporters revealed that multiple isoforms are expressed in mouse circumvallate taste receptor cells.
Rescue studies as well as tissue and isoform specific conditional knock-out experiments like the ones performed for plectin isoforms [3], [29] have been proposed to investigate whether one or multiple isoforms are responsible for the dt disorder [7].
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