For genes with multiple isoforms, the longest transcript was used.
However, native P. jiroveci Msg is in short supply and contains multiple isoforms of this glycoprotein, which complicates immunologic studies of this antigen (23, 24 ).
The observation that several spots matched to this protein supports previous data that there are multiple isoforms of this enzyme [ 31].
Acetyl-CoA synthase and malate dehydrogenase exist as multiple isoforms in this microalga, therefore, we first identified those implicated in the glyoxylate cycle based on the observation that lack of isocitrate lyase (ICL) in a previously identified icl deficient mutant was correlated with specific loss of the other enzymes of the glyoxylate cycle.
Two bands were observed for OsGLP8-12+C′GFP proteinsuggestinging multiple isoforms of this protein.
All these could, at least in part, justifying the conflicting and heterogeneous data of studies revised in this work, which preceded the knowledge of PARK2 alternative splicing and expression of multiple isoforms for this gene.
* Indicates multiple isoforms for this protein but only one is shown in table, # the nucleotide position is referred to UCSC browser human genome build 36.1 and mouse genome build 36.
Besides, the observations that three spots (8, 9, and 10) are matched to TspSP-1, support previous data that there are multiple isoforms of this protein present in the ES fraction [ 38].
Besides the well-known examples of MOMP and FlaA, IlvE, AphC, Cj0355c (CosR, an oxidative stress response regulator), OorC, Cj1419c, Cj1110c, TrxB, GroEL, and Pta were also found to have multiple isoforms in this study (supplementary table S6, Supplementary Material online).
Of course, since the ESCRTIII complex in mammalian cells has six CHMPs, some with multiple isoforms, one explanation for this finding is that CHMPs may be partially redundant.
