When multiple isoforms are present and differentially deadenylated, the expected pattern is segmentation of the GV-MIIpa relative expression plot, but no change in the GV-MII plot (Figure 3).
When multiple isoforms were present, one representative was selected randomly.
In cases where multiple isoforms were present, we chose the longest isoform.
This technique retains the isoform that is most abundant across all clones, when multiple isoforms were present.
When multiple isoforms were present, we removed all but one representative sequence based on the following criteria.
However, if multiple isoforms are present, a sequence read may contain a portion that aligns perfectly with the previously constructed contig and a portion that does not (with the point of divergence being, for example, an exon-exon junction).
As is the case with the Acanthamoeba proteins, the vast majority of γCA proteins from eukaryotes outside of the plant supergroup do not individually conserve all three metal-binding His residues, although all three are often found among different γCAs where multiple isoforms are present.
eIF4E plays a conserved role in initiating protein synthesis, but with multiple eIF4E isoforms present in many organisms, these proteins also adopt specialized functions.
The detection of OXR1 transcripts has also been reported outside of the CNS (Yang et al., 2014); however, by western blot we show here that the brain and spinal cord express the majority of the full-length OXR1 protein, with multiple smaller isoforms present in the CNS and other organs (Supplementary Fig. 1B).
Furthermore, it enables simultaneous detection of multiple different alternative isoforms present in a single sample.
