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The two amplified haemocyanin regions FU f-g and FU g were not concatenated as the presence of multiple isoforms as well as allelic variation did not allow reliable sequence matching.
However, muscle fiber types are distinguished not just by MyHC expression but by the expression of other sarcomeric proteins, most of which also exist as multiple isoforms, as well as by isoforms of intracellular calcium signaling components and levels of metabolic enzymes (Schiaffino and Reggiani, 1996; Pette and Staron, 2000; Schiaffino and Reggiani, 2011).
p63 can be produced in multiple isoforms as the result of transcription from two different promoters and differential splicing.
Proteolysis is unlikely to account for the multiple isoforms as protease inhibitors were included during protein extraction (Section 2).
The NHS gene appears to have multiple isoforms as a result of alternative transcription, but a cellular function for the NHS protein has yet to be defined.
We will refer to reads that map to multiple genes as gene multireads and reads that map to a single gene but multiple isoforms as isoform multireads.
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Circulating adiponectin concentrations are inversely proportional to adiposity [ 5], and adiponectin circulates as multiple isoforms characterized as low, medium, and high molecular weight (HMW), with the HMW isoform being the most abundant and demonstrating the greatest insulin sensitizing properties [ 6, 7].
Both cAMP and PKA are critically regulated by phosphodiesterase (PDE), which has multiple isoforms such as PDE1 and PDE4 [ 9].
Also, our study did not address specific effects of IRF5 alternative splicing isoforms and avoided expression data from exons with multiple isoforms, such as exon 1.
In addition, within a single species, the bombinins occur in multiple isoforms often differing by as little as a single amino acid residue and they are generally C-terminally amidated [ 14].
Triazoles affected the expression of numerous CYP genes in rat liver and testis, including multiple Cyp2c and Cyp3a isoforms as well as other xenobiotic metabolizing enzyme (XME) and transporter genes.
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