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In a typical lipid analysis, precursor scans of phospholipids and multiple ion scans of phosphoinositides were acquired.
Quantification of diacyl (Figure 3B-green) and alkyl-acyl (Figure 3B-blue) species from the multiple ion scans of the phosphoinositides showed that 38±4% of PtdInsP, 15±4% of PtdInsP2 and 49±14% of PtdInsP3 were diacyl lipids.
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MS/MS analysis of target analytes was achieved using a combination of multiple reaction monitoring and precursor ion scans, with the ESI source being operated in the positive ion mode.
Following sample extraction and butylation of amino acids and acylcarnitines, detection and quantitation of acylcarnitine butyl esters was achieved by MS/MS using Waters Quattro Micro tandem mass spectrometers in combination with Waters 1525 μ Binary HPLC Pump using a combination Multiple Reaction Monitoring (MRM) and Parent ion scans of 85 Da.
A targeted profiling scheme was used to quantitatively screen for known small molecule metabolites using multiple reaction monitoring, neutral loss and precursor ion scans.
Briefly, a targeted profiling scheme is used to quantitatively screen for known small molecule metabolites using multiple reaction monitoring, neutral loss and precursor ion scans.
The MS scan was configured to Multiple Ion Detection (MID) to simultaneously measure compounds of different masses.
The kit assay relies on selective derivitization and extraction, and resulting analytes are profiled using pre-defined multiple reaction monitoring (MRM) pairs, neutral loss measurements and precursor ion scans.
Dynamic background subtraction was used prior to ion selection; the four most abundant doubly or triply charged ions were selected for the product ion scans.
Enhance all was turned on for the product ion scans.
All analyses, full scans, product ion scans, and MRM were conducted in positive ESI mode.
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