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However due to multiple internal deletions the streptin ICE-like region present in the genome of the two serotype M1 strains is only ∼10 kb in size.
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B. bronchiseptica complex IV strains (D445, Bbr77, and MO149) have the most divergent fha locus (as low as 92% identity) compared to RB50, while B. pertussis strains have the most divergent fim loci (as low as 84% identity) with gene deletions and multiple internal sequence variations.
Such a bipartite particle system (with two classes of genomes with internal deletions, but consisting of a mutant spectrum, with multiple minority genomes with non-identical internal deletions [6]) can be regarded as a candidate anti-FMD vaccine.
Multiple alignments of nonautonomous elements and their master elements suggested that they originated by internal deletions from master elements (fig. 4 A– C).
and in the case of multiple internal seeds, (22).
Internal deletions were generated using the inverse PCR method.
It has been suggested that alphavirus DI particle RNA with large internal deletions may arise by sequential deletion events [31].
None of these internal deletions leads to the nuclear localization of the Gli2 protein (Fig. 1K N).
Second, Rag-mediated internal deletions in the Bcl11b gene locus were detected in premalignant DKO thymocytes.
Internal deletions in genes hlyA, hapA, rtxA, and lacZ were created as previously described [7], [8].
We describe the use of BRED to construct unmarked gene deletions, in-frame internal deletions, base substitutions, precise gene replacements, and the addition of gene tags.
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