Sentence examples for multiple internal control from inspiring English sources

Vandesompele, J. et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Vandesompele, J. et al Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.

Analysis of SOX internal control weakness disclosures reveals that firms (1) often have multiple internal control weaknesses, (2) frequently have a mix of information technology (IT) and non-IT control weaknesses, and (3) invariably fail to remediate all control weaknesses in the period they are identified.

The method described in GeNorm [42] was used to derive normalization factors from the multiple internal control genes and relative quantification was performed using the 2−ΔΔCt method.

Expression of the genes of interest was normalized by geometric averaging of multiple internal control genes (CPSF6, HNRPM, EEF1A1, RPL11 and RPL13) using the Genorm program [ 66].

Accurate quantification was done by averaging the geometric mean of multiple internal control reference genes [ 47] such as β-Actin, 18S, GAPDH and relative expression was estimated by Relative Expression Software Tool (REST; [ 48]).

The use of multiple internal controls afforded robustness to our analysis.

This method requires a low amount of DNA, uses multiple internal controls and appears sensitive and reproducible.

To ensure that the immunoassay experiments with these chips are reproducible and interpretable, multiple internal controls were spotted on the chip.

Transcript abundance estimates were carried out using the bicolour CATMA microarrays version 5, containing 34,529 spots (including multiple internal controls) allowing, among others, to uniquely interrogate 26,166 nuclear genes (predicted from TAIR and EUGENE algorithms) in A. thaliana [ 22, 35].

In the study period, multiple internal quality controls with various concentrations have been used.