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Both fibrinogen and fibrin also have multiple interacting sites serving as adhesion motifs, including binding sites for α5β1 integrins [ 22], it is therefore also possible that the abundance of fibrin within the hydrogel may also serve to mediate successful attachment and proliferation of hESCs.
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Many target proteins interact with a number of different partners and may possess multiple interaction sites.
TSAd is known to interact with the Src kinase Lck [15] through the Lck SH2- and SH3-domains [22] via multiple interaction sites [20].
It is even possible to design and fabricate ribozymes endowed with multiple interacting effector binding sites.
The problem of multi-site damage and multiple interacting cracks is one experienced by many aircraft manufacturers and operators.
Multiple interacting systems can be designed with separation of concerns.
We used multiple alignment to check whether an amino acid that is deleterious in flies can co-occur with D. melanogaster amino acids at this interacting sites.
Multiple regulatory sites interacting with increased number of trans-factors can achieve increased network connectivity.
Recently, NMR analysis showed that Sic1 indeed exists in an intrinsically disordered state and its multiple phosphorylated sites interact with the single receptor site of Cdc4 in dynamic equilibrium [24], [25].
Among them, 782 single binding site targets interact with 1988 ligands to form 2561 interaction pairs, and other multiple binding sites targets interact with 722 ligands to form 854 interaction pairs.
This phenomenon might have a significant impact in vivo, as DC and T cells interact at multiple sites including the site of entry (mucosal tissue) and lymphoid structures within the infected host.
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