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The levels of the recombinant laccase activity secreted in the culture media were 0.25 and 1 U ml−1 for single and multiple integrations, respectively.
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We observed multiple integrations in most clones.
The weakened URA3 marker ensures multiple integrations of the expression cassette26.
However, one caution: Should you need or require multiple integrations, go cautiously.
Multiple integrations may have required maintenance of the eccDNA molecule through each sequential integration, before being lost.
Many lines in the collection likely contain multiple integrations.
Multiple integrations may have happened in some cases.
Background noise was minimized by acquisition of multiple integrations, the time per integration being limited by detector saturation.
These have involved both multiple integrations of one construct into different locations [ 75] or integration of multiple constructs into one site on the X chromosome [ 13].
Multiple copies could have arisen by multiple integrations of badnaviruses into the host genome or by the single integration of concatemers whose component sequences then diverged.
However, in some of our subclones we do see multiple integrations.
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