From this, we can provide the value of the defect rate by evaluating the multiple integration in (4) numerically, but it is almost impossible if the dimension of x is large.
Multiple integrations were observed in many cases (mean 3±2), and a maximum of 10 independent events were disclosed on six different chromosomes in the male head of family b (Figure 1).
Multiple integrations were seen in many cases (mean 6.1±2.7), and a maximum of 11 independent events were captured in patient 4. VkDTs were detected in the germ-line cells of nine males with active T. cruzi infections and in six infection-free F1 and F2 progeny within the population studied.
Semi-quantitative RT-PCR confirmed the presence of Ppα-DOX-GUS fused transcripts only in Ppα-DOX-GUS-12, probably due to the multiple integration events in Ppα-DOX-GUS-2.
Multiple integrations may have happened in some cases.
Therefore, the second BamHI site of the BamHI fragments must be located within the genomic sequence flanking the integrated plasmid, and detection of more than one BamHI hybridization signal indicates multiple integration events in different areas of the genome.
Isolated U. maydis transformants were tested by southern analysis to assess single or multiple integration events in the ip locus.
In case of the dKO-2 line, an additional band of 1.5 kb was observed, supporting multiple integration events in the same locus.
The finding of a monophyletic clade for placental mammals with samples from several continents requires a single ancient integration with several losses of NIRV signal or multiple integrations of a related virus in unrelated mammal groups (Fig. 6).
Maury, J. et al. EasyCloneMulti: A Set of Vectors for Simultaneous and Multiple Genomic Integrations in Saccharomyces cerevisiae.
