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However, in most lines showing defective germination, segregation was consistent with the multiple integration of DS10 DR2.
Among the URA3 colonies, cells overproducing the Svp26 protein at appropriate levels due to multiple integration of the plasmid at the ura3-52 locus were selected by immunoblotting of the lysates with an anti-Svp26 antiserum.
The increased sensitivity of the AFPEn0.2-TK transfectants, despite minimal transcriptional activity of the AFPEn0.2 DNA in these cells, was probably due to multiple integration of transfected DNA, in which condition cellular and/or plasmid-derived promoters could activate the TK gene.
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The weakened URA3 marker ensures multiple integrations of the expression cassette26.
Weather forecast ensembles, consisting of multiple integrations of dynamical forecast models, are intended to represent effects of initial-condition sensitivity and model structural errors.
In the July, 2010, issue of PLoS Pathogens, volume 6, issue 7, pages 1 13 (http://www.plospathogens.org), Vladimir Belyi, Arnold J. Levine, and Anna Marie Skalka published "Unexpected Inheritance: Multiple Integrations of Ancient Bornavirus and Ebolavirus/Marburgvirus Sequence in Vertebrate Genomes".
Analysis of the genome of an EHOS showed that the EHOS contained multiple integrations of mobile elements.
These have involved both multiple integrations of one construct into different locations [ 75] or integration of multiple constructs into one site on the X chromosome [ 13].
> PALZ39E and PALZ39M cells are MEL cells having multiple integrations of a 15-kbp plasmid (Dietzel et al. 2004).
As suggested above, this result could be due to multiple integrations of the expression cassette or to chromosomal position effects.
Multiple copies could have arisen by multiple integrations of badnaviruses into the host genome or by the single integration of concatemers whose component sequences then diverged.
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