As was the case for heparin, fertilization of the starfish eggs pre-incubated with JAS produced polyspermy, as indirectly shown by the presence of multiple initiation sites of Ca2+ waves that represent loci of sperm interaction (Fig. 7B, arrowheads).
The idea that the heparin-induced polyspermy was linked to the structural alteration of the cortical actin cytoskeleton was strongly supported by the finding that cortical actin hyperpolymerization by JAS also led to polyspermy, as judged by the formation of multiple initiation sites of Ca2+ signals.
The use of multiple initiation codons, including out-of-frame initiation codons has been previously identified for plant viral mRNAs [ 43].
The results revealed the presence of multiple initiation sites on both strands, all of them located within the satellite monomer.
In the case of the presence of multiple initiation codons, the ORF showing the longest CDS was selected.
As we did not score more than one lesion per individual anatomic lobule, to avoid double-counting large or discontinuous lesions, this number likely underestimates the overall PanIN burden in Ptf1a cKO; Kras G12D pancreata given the likelihood of multiple initiation events per lobule.
To further improve the performance of gNMF, we implemented a new multiple initiation strategy.
In B. pertussis populations from multiple countries, initiation of a vaccination program using a Fim2-expressing vaccine strain has led to the significant enrichment of Fim3-expressing isolates (reviewed in [ 38].
Because all epidemiology-trained veterinarians were assigned to regional outbreak containment at multiple foci, initiation of coordinated epidemiologic investigation in the farms was delayed by up to 10 days.
The multi-lobed morphology of amylose-only granules may be explained by abnormal multiple initiations followed by fusions of small granules.
