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Whereas conventional methods identified no multiple infections, real-time PCR found the prevalence of multiple infections to be 35%.
For this reason we limited the analysis of multiple infections to the 13 HR-HPV types targeted by HR-HC2 test.
HPV prevalence data (19 high-risk and 8 low-risk types) were reported for those cases which were positive for HPV DNA, and data analyses included algorithms of multiple infections to estimate type-specific relative contributions.
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On the one hand, this demonstrates the interest of such a simple biochemical test to refine PrP analysis, and on the other hand it raises a question about the existence of different PrPres signatures in the same patient, i.e., different prion strains linked to multiple infections or to variants selected by the host.
None of the hrHPV types showed marked differences in clearance rates when comparing single infections at baseline to multiple infections at baseline (data not shown).
Single restriction enzyme digestion was performed on wsp amplicons to test for multiple infections and to confirm the presence of no more than the two sequence types revealed via clone sequencing.
The fact that multiple infections appear to be common advocates the need to study transmission mechanisms in lice strains carrying several viruses.
Further research with larger numbers of patients and age-matched control groups is needed to determine the real clinical impact of multiple infections and to determine whether the use of real-time PCR prevents unnecessary antibiotic treatment.
In 32% of the February TS samples the RespiFinder-19 assay detected multiple infections, corresponding to results of Fox [ 10], whereas the RVP assay detected no multiple infections.
The importance of multiple infections needs to be elucidated in longitudinal studies with histological confirmation of progressive CIN.
In addition, women with cervical lesions had a higher rate of multiple infections compared to those who had normal cytology.
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