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The idea of a pan-genome for a species suggests that the genomes of multiple independent strains will be required to understand the diversity and complexity of any bacterial "species".
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However, if we can obtain multiple independent mutant strains from our phenotype screen, the statistics of independent selection events will quickly help distinguish the true target genes.
The Tet-on MnSOD and Cu/ZnSOD expression constructs were created by cloning the corresponding cDNAs [58] into the USC1.0 vector [59] and generating multiple independent transformant strains.
For such organisms, phenotype sequencing can open up a fast path for directly identifying a phenotype's genetic causes, for any phenotype where a good screen exists for generating multiple independent mutant strains.
The results shown are representative of multiple independent suppressor strains.
Southern analysis was performed to confirm single-copy reintegration into the correct locus, and multiple independent reintegrant strains were analysed and data pooled.
These constructs were introduced into Drosophila using P element mediated transformation and multiple independent transgenic strains were generated for each construct.
Analysis of multiple independent disruption mutant strains for each candidate vic allele failed to find any obvious changes in growth, colony morphology, sporulation, or virulence (data not shown).
In our analysis of the emm59 GAS epidemic in Canada, we rapidly identified several polymorphisms that arose multiple, independent times in the strains studied.
In this work we have generated a synthesis of the proteomic datasets we produced from multiple independent comparisons of Brucella strains either grown in media or retrieved from infected host cells.
This study identifies the genetic regulation of basal and dexamethasone-induced levels of the neurosteroid DOC and links the genetic variation of this steroid with behavioral phenotypes and gene expression profiles previously determined across the BXD strains by multiple independent labs.
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