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Previous studies have suggested that the Ebola outbreaks have stemmed from multiple, independent introductions of the virus, which had remained dormant in certain places.
Both herbarium records [28] and recent molecular studies [29], [30] based on nuclear microsatellite markers suggested multiple independent introductions from North America into France.
For datasets where individual values correspond to completely unconnected events (e.g. epidemiological surveillance of multiple independent introductions of a disease, or many other biological observations), the effects of two forms of under-reporting were assessed.
Gladieux et al. [35] suggested that Eastern European populations did not originate from the earlier established French populations but rather represented multiple independent introductions from other sources, or introductions from an unidentified highly diverse native population.
Each clade belonged to a separate phylogenetic lineage supporting a scenario of multiple independent introductions to Albania of subtype B from different geographic areas (the ML genealogy displaying full sequence names is given in supplemental Figure S1B).
Although a number of important recent studies have explored intraspecific admixture following multiple independent introductions of animal taxa [16], [20], there are few cases that describe interspecific hybridization between multiple introduced species [21], [22].
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In contrast, our findings here concerning CRF01_AE showed multiple independent and approximately concurrent introductions into China, followed by population-specific and region-specific transmission through predominantly sexual routes (Fig. 2).
The genetic diversity among the swine-origin influenza A virus isolates from Fair E is probably a result of multiple independent influenza A virus introductions into the swine population; Fair E hosts a larger number of swine (>800), representing a larger geographic area than the other fairs in the study.
To determine whether Submariner_Ps1 proliferation occurred within the P. salinus genome, or whether the multiple Submariner_Ps1 insertions resulted from independent introductions from a different genome (e.g. a viral host), we used 100 bp of immediately flanking sequence from all 30 Submarinsertionsnsertoons to query both amoeba and pandoravirus genomes using BLASTn.
A decade ago, simultaneous introduction via co-bombardment of multiple independent DNA molecules (genes) into rice cells was described.
Their adaptations to the subterranean environment is in accordance with the adaptive shift hypothesis [ 19], that invokes multiple independent colonisations of the subterranean medium and continued survival of the epigean ancestors (see Introduction).
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