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Multiple independent clones were obtained as ORF1-only (ORF1) or ORF2-only (ORF2) MAEBL expression mutants.
Transformants were selected on BSK-II plates containing 200 µg/ml kanamycin and 40 µg/ml gentamicin and multiple independent clones each were expanded in selective liquid BSK-II [11].
Dissection across oesophagectomy specimens demonstrated marked clonal heterogeneity, with multiple independent clones present.
Multiple independent clones were isolated and sequenced in order to avoid clones with PCR-induced mutations.
Multiple independent clones were isolated and expanded in the complete growth medium containing G418.
To avoid the potential error in PCR or sequencing, each nucleotide sequence was determined by multiple independent clones.
Similar(40)
Following transfection, multiple-independent clones expressing DC-SCRIPT (MCF7SC) upon stimulation with doxycycline or the empty control construct were isolated (MCF7EV) (data not shown).
Finally, all edits were inferred from clean sequence reads, generated from both strands of a clone, and most were also validated by obtaining the same sequence from multiple independent cDNA clones (Table 1).
Multiple independent isogenic clones were analyzed with identical findings.
We obtained multiple independent cDNA clones coding for BTBD1 and BTBD2, two uncharacterized, BTB domain-containing, kelch-like proteins.
The demonstration of multiple competing independent clones and the identification of the origin of Barrett's oesophagus from the oesophageal gland squamous duct, structures present throughout the length of the oesophagus, allows the development of a new model of clonal evolution in Barrett's oesophagus.
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