The β,γ-modified tTTPs undergo a single incorporation event with HIV RT but undergo multiple incorporations to form full-length product with engineered thermophilic polymerases.
Multiple incorporations of 7-chloro-7-deaza-2′-deoxyguanosine in place of 2′-deoxyguanosine have been performed into a triple helix-forming oligodeoxyribonucleotide involving a run of six contiguous guanines designed to bind in a parallel orientation relative to the purine strand of the DNA target.
Single and multiple incorporations of stereochemically pure modified dinucleoside-phosphoramidates involving substituent groups ending with bis-hydroxyethyl and bis-aminoethyl groups have been performed into pyrimidic triple helix-forming oligo-2′-deoxyribonucleotides designed to bind parallel to the purine strand of the DNA target.
The yak and mithan phylogenies also contain long internal branches, suggesting multiple incorporations of divergent haplotypes into the domestic pool.
Since these regions require multiple incorporations of identical nucleotides, this increases the chance of non-linearity between the signal intensity and homopolymer length, explaining the higher indel error rate of the Ion Torrent PGM.
The extent of the fully extended products, which is indicative of the polymerase activity in the multiple nucleotide incorporation assays, was determined.
Although proteins travel in the cell and can coexist in multiple compartments, incorporation of subcellular localization data helps eliminate many false negative interactions.
The lack of edited sites in cox3 and rps13 in some lineages of Amaryllidaceae and Iridaceae has been explained by multiple independent incorporation events of processed paralogs into the mitochondrial genome, replacing the original gene [ 18].
Here, we describe a newly designed, chemically versatile protocol mediating single- or multiple-isotope incorporation into aziridines via a one-pot, three-component, two-step process.
Of course, multiple site incorporations of tfmF and 19F-NMR will be required to reflect the uniform changes of the sample conditions, e.g. acidification, viscosity changes or protein degradation.
